Transfection of Escherichia coli by Mu DNA

Kahmann, R., Kamp, D., Zipser, D. (1976) Transfection of Escherichia coli by Mu DNA. Mol Gen Genet, 149 (3). pp. 323-8.



Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

Item Type: Paper
Subjects: organism description > bacteria > escherichia coli
CSHL Authors:
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: 1976
Date Deposited: 22 Feb 2019 15:41
Last Modified: 22 Feb 2019 15:41
PMCID: PMC256058

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving