Burbulis, Ian E., Wierman, Margaret B., Wolpert, Matt, Haakenson, Mark, Lopes, Maria-Beatriz, Schiff, David, Hicks, James, Loe, Justin, Ratan, Aakrosh, McConnell, Michael J. (July 2018) Improved molecular karyotyping in Glioblastoma. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 11. pp. 16-26. ISSN 0027-5107
Abstract
Uneven replication creates artifacts during whole genome amplification (WGA) that confound molecular karyotype assignment in single cells. Here, we present an improved WGA recipe that increased coverage and detection of copy number variants (CNVs) in single cells. We examined serial resections of glioblastoma (GBM) tumor from the same patient and found low-abundance clones containing CNVs in clinically relevant loci that were not observable using bulk DNA sequencing. We discovered extensive genomic variability in this class of tumor and provide a practical approach for investigating somatic mosaicism
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics diseases & disorders > cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants diseases & disorders > cancer > cancer types > glioblastoma diseases & disorders > cancer > cancer types |
| CSHL Authors: | |
| Depositing User: | Matthew Dunn |
| Date: | 8 July 2018 |
| Date Deposited: | 30 Jul 2018 15:48 |
| Last Modified: | 07 Feb 2024 18:37 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/37082 |
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