Evans, K. C., Benomar, S., Camuy-Velez, L. A., Nasseri, E. B., Wang, X., Neuenswander, B., Chandler, J. R. (January 2018) Quorum-sensing control of antibiotic resistance stabilizes cooperation in Chromobacterium violaceum. The ISME Journal, 12 (5). pp. 1263-1272. ISSN 1751-7362
Abstract
Many Proteobacteria use quorum sensing to regulate production of public goods, such as antimicrobials and proteases, that are shared among members of a community. Public goods are vulnerable to exploitation by cheaters, such as quorum sensing-defective mutants. Quorum sensing- regulated private goods, goods that benefit only producing cells, can prevent the emergence of cheaters under certain growth conditions. Previously, we developed a laboratory co-culture model to investigate the importance of quorum-regulated antimicrobials during interspecies competition. In our model, Burkholderia thailandensis and Chromobacterium violaceum each use quorum sensing-controlled antimicrobials to inhibit the other species' growth. Here, we show that C. violaceum uses quorum sensing to increase resistance to bactobolin, a B. thailandensis antibiotic, by increasing transcription of a putative antibiotic efflux pump. We demonstrate conditions where C. violaceum quorum-defective cheaters emerge and show that in these conditions, bactobolin restrains cheaters. We also demonstrate that bactobolin restrains quorum-defective mutants in our co-culture model, and the increase in antimicrobial-producing cooperators drives the C. violaceum population to become more competitive. Our results describe a mechanism of cheater restraint involving quorum control of efflux pumps and demonstrate that interspecies competition can reinforce cooperative behaviors by placing constraints on quorum sensing-defective mutants.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > Bacterial Infections diseases & disorders diseases & disorders > Bacterial Infections > Antibiotic Resistance organism description > bacteria |
| CSHL Authors: | |
| Communities: | CSHL labs > Ware lab |
| Depositing User: | Matt Covey |
| Date: | 26 January 2018 |
| Date Deposited: | 05 Feb 2018 17:10 |
| Last Modified: | 20 Feb 2024 18:48 |
| PMCID: | PMC5932061 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/36067 |
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