Triple S-Phase Labeling of Dividing Stem Cells

Podgorny, O., Peunova, N., Park, J. H., Enikolopov, G. (February 2018) Triple S-Phase Labeling of Dividing Stem Cells. Stem Cell Reports, 10 (2). pp. 615-626. ISSN 2213-6711

Abstract

Marking replicating DNA with multiple labels presents the possibility of revealing new features and mechanisms of DNA synthesis and cell division; however, progression beyond double labeling has been hampered by cross-reactivity of label detection and scarcity of appropriate labels. Here, we present a method for triple S-phase labeling of the dividing cells, with a fourth label used to mark cells actively engaged in cell-cycle progression (e.g., using Ki67) or to phenotype the dividing cells or their progeny (e.g., using a GFP-expressing lineage reporter transgene). We apply this method to determine the parameters of neural stem cell division in the adult brain, to birth date up to four cohorts of dividing cells, and to reveal patterns of stem cell division in non-neural tissues.

Item Type:	Paper
Uncontrolled Keywords:	S-phase labeling adult neurogenesis cell cycle dentate gyrus intestinal stem cells neural stem cells proliferation stem cell maintenance subventricular zone thymidine analogs
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurogenesis organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:	Enikolopov, Grigori N. Park, June-Hee
Communities:	CSHL labs > Enikopolov lab
Depositing User:	Matt Covey
Date:	13 February 2018
Date Deposited:	26 Jan 2018 20:28
Last Modified:	28 Jan 2019 20:46
PMCID:	PMC5830949
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/35975

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