Gay, Helen, Kaufmann, Berwind Petersen (October 1950) The corneal epithelium as a source of mammalian somatic mitosis. Stain Technology, 25 (4). pp. 209-216. ISSN 0038-9153
Abstract
Dividing cells that occur abundantly in the lowermost layers of the corneal epithelium of various laboratory mammals provide a reliable and readily procurable source of mitotic figures for studies of chromosome number and form. Preparations may be secured quickly by fixing and staining entire eyes in an orcein-alcohol-acetic-acid mixture, or by fixing in acetic-alcohol and then staining with orcein or with leucobasic f uchsin as used in the Feulgen technic. For cytological examination of the epithelial cells and for determination of mitotic rates, the cornea is dissected from the eye and mounted as a flat preparation. Squashes of the epithelial cells are useful if mitotic counts are not required.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals *Cell Division *Chromosomes *Coloring Agents *Epithelium *Epithelium, Corneal *Mitosis *Staining and Labeling *Stains and staining |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis |
| CSHL Authors: | |
| Communities: | The Carnegie Institution Department of Genetics |
| Depositing User: | Matt Covey |
| Date: | October 1950 |
| Date Deposited: | 23 Jan 2018 15:53 |
| Last Modified: | 23 Jan 2018 15:53 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35936 |
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