Sturm, R. A., Das, G., Herr, W. (December 1988) The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain. Genes Dev, 2 (12A). pp. 1582-99. ISSN 0890-9369
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Abstract
The octamer motif ATGCAAAT is recognized indistinguishably by two mammalian transcription factors: one that is expressed ubiquitously and referred to here as Oct-1, and another, Oct-2, that is expressed in lymphoid cells. We report the cDNA cloning of the human oct-1 gene, which encodes Oct-1, by screening lambda gt11 recombinant phage in situ for octamer motif-specific DNA binding. One lambda gt11 recombinant expressed a beta-galactosidase-octamer-binding fusion protein with a DNA-binding specificity indistinguishable from human HeLa cell Oct-1 protein. As expected for a ubiquitously expressed protein, Oct-1 mRNA is expressed in all five human and two mouse cell lines tested. Polyclonal rabbit antiserum raised against the beta-galactosidase fusion protein shows that the DNA-binding domains of Oct-1 and Oct-2 proteins are related antigenically. Deletion analysis of the 743-amino-acid-long oct-1 open reading frame shows that the DNA-binding activity lies within a central highly charged domain of 160 amino acids. Comparison of the Oct-1 and Oct-2 sequences reveals that this domain is nearly identical between the two proteins. Highly similar domains are also present in the pituitary-specific transcription factor Pit-1 and the Caenorhabditis elegans unc-86 cell lineage gene product (see Herr et al. 1988). Within this shared POU (Pit-1, Oct-1 and Oct-2, unc-86) domain (pronounced 'pow') lie two subdomains: a POU-related homeo box and a POU-specific box. The Oct-1 protein is unique among the POU-related proteins and other homeo box proteins because it is expressed ubiquitously.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Bacteriophage lambda/genetics Base Sequence Binding Sites Cell Line Cloning, Molecular Comparative Study DNA-Binding Proteins/*genetics Genes, Homeobox *Genes, Structural Humans Immunoblotting Lymphoid Tissue/cytology Mice Molecular Sequence Data Nuclear Proteins/*genetics Recombinant Fusion Proteins/genetics/immunology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Transcription Factors/genetics beta-Galactosidase/genetics |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design organism description > animal bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Gail Sherman |
| Date: | December 1988 |
| Date Deposited: | 14 Sep 2017 20:08 |
| Last Modified: | 03 Nov 2017 21:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35219 |
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