Simian virus 40 Revertant Enhancers Exhibit Restricted Host Ranges for Enhancer Function

Shepard, A., Clarke, J., Herr, W. (September 1988) Simian virus 40 Revertant Enhancers Exhibit Restricted Host Ranges for Enhancer Function. Journal of Virology, 62 (9). pp. 3364-3370. ISSN 0022-538X



We have assayed the cell-specific activity of a matched set of four enhancers found in viral revertants derived from simian virus 40 (SV40) enhancer mutants. These enhancers all contain 71-base-pair duplication that span identical regions or, in one case, the same region shifted by 2 nucleotides. The four enhancers differ, however, in that each one either carriers a different wild-type pair of the genetically defined SV40 enhancer A, B, or C elements, with the other two elements mutated, or carries all three elements mutated. The three enhancers carrying two copies of wild-type element effectively enhance transcription in CV-1 and HeLa cells, but only the enhancer containing a duplicated wild-type C element exhibits activity in NIH 3T3 cells. These results show that the ability of the A, B, and C elements to compensate for one another is cell specific and that selection for enhancer function in one cell type can generate enhancers with different cell-specific activities. These results are consistent with the hypothesis that tandem duplication of multiple distinct enhancer elements, as in wild-type strains of SV40 (e.g., the 72-base-pair repeat), has the property of expanding the host range of an enhancer.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus > SV40
Communities: CSHL labs > Herr lab
Highlight: Herr, Winship
Depositing User: Gail Sherman
Date: September 1988
Date Deposited: 15 Sep 2017 16:03
Last Modified: 15 Sep 2017 16:03
PMCID: PMC253459
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