Shepard, A., Clarke, J., Herr, W. (September 1988) Simian virus 40 Revertant Enhancers Exhibit Restricted Host Ranges for Enhancer Function. Journal of Virology, 62 (9). pp. 3364-3370. ISSN 0022-538X
Abstract
We have assayed the cell-specific activity of a matched set of four enhancers found in viral revertants derived from simian virus 40 (SV40) enhancer mutants. These enhancers all contain 71-base-pair duplication that span identical regions or, in one case, the same region shifted by 2 nucleotides. The four enhancers differ, however, in that each one either carriers a different wild-type pair of the genetically defined SV40 enhancer A, B, or C elements, with the other two elements mutated, or carries all three elements mutated. The three enhancers carrying two copies of wild-type element effectively enhance transcription in CV-1 and HeLa cells, but only the enhancer containing a duplicated wild-type C element exhibits activity in NIH 3T3 cells. These results show that the ability of the A, B, and C elements to compensate for one another is cell specific and that selection for enhancer function in one cell type can generate enhancers with different cell-specific activities. These results are consistent with the hypothesis that tandem duplication of multiple distinct enhancer elements, as in wild-type strains of SV40 (e.g., the 72-base-pair repeat), has the property of expanding the host range of an enhancer.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organism description > virus > SV40 |
| Communities: | CSHL labs > Herr lab |
| Highlight: | Herr, Winship |
| Depositing User: | Gail Sherman |
| Date: | September 1988 |
| Date Deposited: | 15 Sep 2017 16:03 |
| Last Modified: | 15 Sep 2017 16:03 |
| PMCID: | PMC253459 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35212 |
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