Rice, A. P., Mathews, M. B. (April 1988) Transcriptional but not translational regulation of HIV-1 by the tat gene product. Nature, 332 (6164). pp. 551-3. ISSN 0028-0836
Abstract
Human immunodeficiency virus-1 (HIV-1), which causes AIDS (acquired immune deficiency syndrome), possesses an essential gene, tat, whose product, acting through the long terminal repeat (LTR) sequences of HIV-1, activates viral genes and replication. The mechanism by which tat trans-activates HIV genes is unclear. Some studies have reported that an increase in messenger RNA accumulation directed by the HIV-1 LTR can explain the action of tat, but others suggest that this increase in mRNA levels can only partially explain trans-activation, and that translational control mechanisms may also be involved. To test those possibilities we have established an efficient adenovirus system for delivering the HIV-1 LTR attached to a reporter gene (chloramphenicol acetyltransferase; CAT) into cells and monitoring its activity. The HIV-1 LTR expressed from this adenovirus responds to trans-activation in a HeLa cell line constitutively expressing the tat protein by increasing the transcription rate of the HIV-1 LTR and the accumulation of mRNA encoding CAT. In this system the translational efficiency of this CAT mRNA in the cell is unaffected by the presence of tat.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenoviridae/genetics *Gene Expression Regulation Gene Products, tat Genetic Vectors HIV/*genetics Hela Cells Protein Biosynthesis RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Recombinant Fusion Proteins/biosynthesis Research Support, U.S. Gov't, P.H.S. Retroviridae Proteins/*physiology Transcription Factors/*physiology *Transcription, Genetic Virus Replication |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 7 April 1988 |
| Date Deposited: | 15 Sep 2017 15:20 |
| Last Modified: | 15 Sep 2017 15:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35209 |
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