Rice, A. P., Mathews, M. B. (June 1988) Trans-activation of the human immunodeficiency virus long terminal repeat sequences, expressed in an adenovirus vector, by the adenovirus E1A 13S protein. Proc Natl Acad Sci U S A, 85 (12). pp. 4200-4. ISSN 0027-8424
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Abstract
The human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR) sequences were inserted into adenovirus in place of the E1 region. The HIV-1 LTR contained in this recombinant adenovirus responds to trans-activation by tatIII in a HeLa cell line constitutively expressing that HIV-1 gene product. In addition, the HIV-1 LTR is activated by the adenovirus E1A 13S, but not 12S or 9S, gene product when it is supplied in trans by a coinfecting wild-type adenovirus. The Rous sarcoma virus LTR, in a similar recombinant adenovirus, is insensitive to tatIII but is also trans-activated by the E1A 13S protein. The action of the 13S E1A and tatIII proteins are additive for the HIV-1 LTR in the context of adenovirus and they appear to act at the transcriptional level. As in HeLa cells, the adenovirus-borne HIV-1 LTR is inactive in the absence of a trans-activator in H9 and Jurkat cells, two human leukemic T-cell lines. This suggests that recombinant adenoviruses have diagnostic potential for the detection of trans-activators of the HIV-1 LTR that are present in circulating human lymphocytes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Adenoviruses, Human/*genetics Antigens, Viral, Tumor Cell Line *Gene Expression Regulation Genes, Structural *Genes, Viral Genetic Vectors HIV/*genetics Hela Cells/metabolism Humans Oncogene Proteins, Viral/*metabolism Repetitive Sequences, Nucleic Acid Research Support, U.S. Gov't, P.H.S. Viral Proteins/genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > E1A protein diseases & disorders > viral diseases > HIV organism description > virus > adenovirus |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | June 1988 |
| Date Deposited: | 15 Sep 2017 14:14 |
| Last Modified: | 09 Nov 2017 20:48 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35208 |
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