Molecular Studies of Hhv-6

Josephs, S. F., Ablashi, D. V., Salahuddin, S. Z., Kramarsky, B., Franza, B. R., Pellett, P., Buchbinder, A., Memon, S., Wongstaal, F., Gallo, R. C. (September 1988) Molecular Studies of Hhv-6. Journal of Virological Methods, 21 (1-4). pp. 179-190. ISSN 0166-0934

DOI: 10.1016/0166-0934(88)90064-X


Methods for the purification of enveloped HHV-6 virions and the viral DNA are presented. The viral genome is estimated to be 170,000 base pairs in size and does not appear to contain inversions due to absence of submolar bands by restriction enzyme analyses. The genomes of two independent HHV-6 isolates, HHV-6GS and HHV-6Z29, showed restriction enzyme site pleomorphism. Large scale purification of enveloped HHV-6 was achieved by continuous flow centrifugation utilizing sucrose gradients, DNAse 1 treatment and banding on 10-30% Dextran T10 gradients. The viral proteins were visualized on high resolution two dimensional polyacrylamide gels and the proteins recognized by serum antibody from patient GS were detected by HR2D Western blot analysis and radioimmunoprecipitation assay. The major antigenic proteins were 200, 120, 80, 72, 30 and 19 kDa.

Item Type: Paper
Subjects: diseases & disorders > viral diseases
organism description > virus > herpes simplex virus
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: September 1988
Date Deposited: 12 Oct 2017 17:32
Last Modified: 12 Oct 2017 17:32
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