Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons

Alpert, S., Hanahan, D., Teitelman, G. (April 1988) Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell, 53 (2). pp. 295-308. ISSN 0092-8674

Abstract

Insulin appears in the developing mouse pancreas at embryonic day 12 (e12). Transgenic mice harboring three distinct hybrid genes utilizing insulin gene regulatory information first express the transgene product two days earlier, at e10, in a few cells of the pancreatic bud. Throughout development and postnatal life, all of the insulin-producing (beta) cells coexpress the hybrid insulin gene. In addition, islet cells containing glucagon, somatostatin, pancreatic polypeptide, and the neuronal enzyme tyrosine hydroxylase coexpress the transgene when they first arise. Similarly, coexpression of these normally distinct islet cell markers occurs during differentiation of the four endocrine cell types. The transgene product also appears transiently during embryogenesis in cells of the neural tube and in neural crest. The results suggest a common precursor for the endocrine cells of the pancreas. Moreover, they imply a relationship between neural and pancreatic endocrine tissue.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Differentiation Female Genes, Regulator *Genes, Structural Gestational Age Insulin/*genetics Islets of Langerhans/cytology/*embryology Male Mice Mice, Transgenic Neurons/cytology/*physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Subjects: organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > pancreatic stellate cells
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 22 April 1988
Date Deposited: 10 Nov 2017 17:02
Last Modified: 10 Nov 2017 17:02
Related URLs:
URI: https://repository.cshl.edu/id/eprint/35109

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