Two distinct cellular phosphoproteins bind to the c-fos serum response element

Ryan, W. A., Franza, B. R., Gilman, M. Z. (June 1989) Two distinct cellular phosphoproteins bind to the c-fos serum response element. Embo J, 8 (6). pp. 1785-92. ISSN 0261-4189



Induction of c-fos transcription by serum growth factors requires a 20 bp DNA sequence termed the serum response element (SRE). The SRE is a binding site for a nuclear protein, serum response factor (SRF). There is as yet no direct evidence that links SRF activity to SRE function. We show here that cells contain a second specific SRE-binding protein. It is distinguished from SRF by its size, chromatographic properties, binding specificity and contacts with nucleotides in the SRE. The presence in cells of two distinct proteins that can bind independently to the SRE suggests that the multiple functions of the SRE may be mediated by distinct proteins.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites DNA Probes DNA-Binding Proteins/metabolism Humans Mice Phosphoproteins/*metabolism Phosphorylation Proto-Oncogene Proteins/genetics/*metabolism Proto-Oncogene Proteins c-fos Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
Communities: CSHL labs
Depositing User: Gail Sherman
Date: June 1989
Date Deposited: 23 Jun 2017 18:32
Last Modified: 23 Jun 2017 18:32
PMCID: PMC401023

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