The Ad5 12S growth factor induces F9 cell proliferation and differentiation

Quinlan, M. P. (August 1989) The Ad5 12S growth factor induces F9 cell proliferation and differentiation. Oncogene, 4 (8). pp. 1051-5. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/2569707

Abstract

The 12S gene of the E1A region of adenovirus induces the production of a growth factor that is necessary for the immortalization of primary epithelial cells. This growth factor is able to replace the strict serum requirement of F9 cells, a teratocarcinoma-derived embryonal carcinoma cell line. Furthermore, the growth factor induces a morphological alteration in the F9 cells such that they resemble visceral endoderm cells and express the surface antigens, SSEA-1 and SSEA-3, characteristic of these differentiated cells.

Item Type: Paper
Uncontrolled Keywords: Adenoviruses, Human/*physiology Animals Antigens, CD15 Antigens, Surface/analysis Cell Differentiation Cell Division Fluorescent Antibody Technique Glycolipids/analysis Growth Substances/genetics/*pharmacology In Vitro Mice Teratoma/*pathology Tumor Cells, Cultured
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell differentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: August 1989
Date Deposited: 26 Jun 2017 19:30
Last Modified: 26 Jun 2017 19:30
URI: https://repository.cshl.edu/id/eprint/34895

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving