O'Malley, R. P., Duncan, R. F., Hershey, J. W., Mathews, M. B. (January 1989) Modification of protein synthesis initiation factors and the shut-off of host protein synthesis in adenovirus-infected cells. Virology, 168 (1). pp. 112-8. ISSN 0042-6822
Abstract
A substantial body of data, largely derived from study of cell extracts, indicates that protein synthesis in adenovirus-infected cells requires VA RNAI at late times of infection to prevent the activation of a protein kinase known as DAI, and the consequent phosphorylation of the alpha-subunit of initiation factor eIF-2. To verify this conclusion, we have measured the steady-state levels of eIF-2 alpha phosphorylation in cells infected with wild-type virus (Ad2) and a mutant that produces no VA RNAI (Ad5dl331). Consistent with the proposed mechanism, the alpha-subunit was very highly phosphorylated (approximately 90%) at late times of infection with Ad5dl331. Surprisingly, eIF-2 alpha phosphorylation also increased (to approximately 30%) at late times of infection with Ad2, suggesting that VA RNA and DAI might be involved in the selective translation of viral mRNA and the shut-off of host cell protein synthesis during the late phase. In agreement with this model, host protein synthesis shut-off is defective in cells expressing low levels of DAI.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenoviruses, Human/enzymology/genetics/*metabolism Autoradiography Electrophoresis, Polyacrylamide Gel Eukaryotic Initiation Factor-2 Hela Cells Humans Immunoblotting Isoelectric Focusing Mutation Peptide Initiation Factors/*metabolism Phosphorylation *Protein Biosynthesis Protein Kinases/analysis Proteins/*metabolism RNA, Viral/physiology Research Support, U.S. Gov't, P.H.S. eIF-2 Kinase |
| Subjects: | organism description > virus > adenovirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | January 1989 |
| Date Deposited: | 23 Jun 2017 20:16 |
| Last Modified: | 23 Jun 2017 20:16 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34889 |
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