Properties of the DNA-binding domain of the simian virus 40 large T antigen

McVey, D., Strauss, M., Gluzman, Y. (December 1989) Properties of the DNA-binding domain of the simian virus 40 large T antigen. Molecular & Cellular Biology, 9 (12). pp. 5525-36. ISSN 1098-5549

DOI: 10.1128/MCB.9.12.5525


T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding domain is at or near amino acid 246; furthermore, the maximum of these DNA-binding activities coincides with a narrow C-terminal boundary, spanning 4 amino acids (246 to 249) and declines sharply in proteins with C termini which differ by a few (4 to 10) amino acids; (ii) a polypeptide spanning residues 132 to 246 of Tag is an independent domain responsible for origin-specific DNA binding and presumably for single-stranded DNA binding; and (iii) a comparison of identical N-terminal fragments of Tag purified from mammalian and bacterial cells revealed differential specificity and levels of activity between the two sources of protein. A role for posttranslational modification (phosphorylation) in controlling the DNA-binding activity of Tag is discussed.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence *Antigens, Polyomavirus Transforming Base Sequence Chromosome Deletion *DNA Replication DNA, Single-Stranded/genetics/metabolism DNA, Viral/genetics/*metabolism DNA-Binding Proteins/*metabolism Genes, Structural, Fungal Hela Cells/metabolism Humans Molecular Sequence Data Nucleic Acid Heteroduplexes/genetics/*metabolism Oligonucleotide Probes/chemical synthesis Plasmids Recombination, Genetic Research Support, U.S. Gov't, P.H.S. Simian virus 40/genetics/*immunology Templates, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > animal > mammal > primates > monkey
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: December 1989
Date Deposited: 27 Jun 2017 18:37
Last Modified: 27 Jun 2017 18:37
PMCID: PMC363723
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving