McVey, D., Strauss, M., Gluzman, Y. (December 1989) Properties of the DNA-binding domain of the simian virus 40 large T antigen. Molecular & Cellular Biology, 9 (12). pp. 5525-36. ISSN 1098-5549
Abstract
T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding domain is at or near amino acid 246; furthermore, the maximum of these DNA-binding activities coincides with a narrow C-terminal boundary, spanning 4 amino acids (246 to 249) and declines sharply in proteins with C termini which differ by a few (4 to 10) amino acids; (ii) a polypeptide spanning residues 132 to 246 of Tag is an independent domain responsible for origin-specific DNA binding and presumably for single-stranded DNA binding; and (iii) a comparison of identical N-terminal fragments of Tag purified from mammalian and bacterial cells revealed differential specificity and levels of activity between the two sources of protein. A role for posttranslational modification (phosphorylation) in controlling the DNA-binding activity of Tag is discussed.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence *Antigens, Polyomavirus Transforming Base Sequence Chromosome Deletion *DNA Replication DNA, Single-Stranded/genetics/metabolism DNA, Viral/genetics/*metabolism DNA-Binding Proteins/*metabolism Genes, Structural, Fungal Hela Cells/metabolism Humans Molecular Sequence Data Nucleic Acid Heteroduplexes/genetics/*metabolism Oligonucleotide Probes/chemical synthesis Plasmids Recombination, Genetic Research Support, U.S. Gov't, P.H.S. Simian virus 40/genetics/*immunology Templates, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > animal > mammal > primates > monkey organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | December 1989 |
| Date Deposited: | 27 Jun 2017 18:37 |
| Last Modified: | 27 Jun 2017 18:37 |
| PMCID: | PMC363723 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34880 |
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