Laspia, M. F., Rice, A. P., Mathews, M. B. (October 1989) HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation. Cell, 59 (2). pp. 283-92. ISSN 0092-8674
Abstract
We studied regulation of human immunodeficiency virus-1 (HIV-1) transcription by Tat and, for comparative purposes, by the adenovirus E1A protein. These two trans-activators exerted different effects. Two classes of HIV-1-promoted cytoplasmic RNA were detected, one class corresponding to full-length transcripts and the other to transcripts ending 55 and 59 nucleotides from the transcription start. Tat increased the level of the full-length class only, whereas E1A increased the levels of both classes of RNA. We also measured the effects of Tat and E1A on RNA synthesis rates. Without trans-activators, HIV-1-directed transcription was relatively weak and exhibited a marked polarity. Both Tat and E1A dramatically increased promoter-proximal transcription, while only Tat suppressed transcriptional polarity. Mutations in the TAR element did not influence basal transcription rates or the response to E1A, but eliminated trans-activation by Tat. We propose that Tat acts through TAR to increase initiation complex formation on the HIV-1 promoter and to stabilize complexes during elongation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Adenoviruses, Human/genetics Comparative Study DNA-Binding Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Gene Expression Gene Products, tat/*metabolism Genes, Viral HIV-1/*genetics Hela Cells/metabolism Humans Models, Genetic Mutation Oncogene Proteins, Viral/metabolism Plasmids RNA, Viral/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Trans-Activators/*metabolism *Transcription, Genetic |
| Subjects: | diseases & disorders > viral diseases > HIV |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Gail Sherman |
| Date: | 20 October 1989 |
| Date Deposited: | 28 Jun 2017 20:00 |
| Last Modified: | 28 Jun 2017 20:00 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34870 |
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