Lanning, B. R., Vakoc, C. R. (April 2017) Single-minded CRISPR screening. Nat Biotechnol, 35 (4). pp. 339-340. ISSN 1087-0156
Abstract
ooled CRISPR knockout screening is a powerful technique for interrogating the biological function of genes in a non-biased manner. It can, however, only identify genes that influence cellular growth or viability phenotypes or alter the expression of a limited number of specific markers detected using antibodies or fluorescent protein reporters1, 2. Four recent studies published in Cell3, 4, 5 and Nature Methods6 now describe methods (independently termed Perturb-Seq3, 4, CRISP-seq5, or CROP-seq6) that combine pooled CRISPR screening with single-cell RNA-seq analysis, thereby linking each gene knockout with its effect on the expression level of any transcribed gene. These methods expand the resolving power of genetic screens in mammalian cells by not only increasing the number of potential readouts, but also enabling the investigation of the cell-to-cell differences in the effects of genetic knockout. They are therefore likely to have far-reaching applications for the elucidation of biological circuits.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > CRISPR-Cas9 |
| CSHL Authors: | |
| Communities: | CSHL labs > Vakoc lab CSHL Cancer Center Program > Gene Regulation and Cell Proliferation |
| Depositing User: | Matt Covey |
| Date: | 11 April 2017 |
| Date Deposited: | 20 Apr 2017 15:52 |
| Last Modified: | 12 Jun 2018 19:31 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34577 |
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