A method for making aceto-carmin smears permanent

McClintock, Barbara (April 1929) A method for making aceto-carmin smears permanent. Stain Technology, 4 (2). pp. 53-56.

URL: http://www.tandfonline.com/doi/abs/10.3109/1052029...
DOI: 10.3109/10520292909115586


Anthers are collected and placed in a solution of 1 part acetic acid to 3 parts of absolute alcohol. The contents of the anther are squeezed out on a slide in a drop of Belling's iron-aceto-carmin solution and a cover glass placed over the drop. Care should be taken to remove all anther walls and flower parts. Heat the slide over an alcohol flame for a second, repeating 4 or 5 times. Place the slide in a petri dish filled with a 10% solution of acetic acid. When the cover glass has risen away from the slide gently remove the cover glass and place in a Coplin jar containing equal parts of alcohol and acetic acid. Likewise, place the slide in this solution. Run both cover and slide thru the following solutions: 1 part acetic acid to 3 parts absolute alcohol, 1 part acetic acid to 9 parts absolute alcohol, absolute alcohol and finally equal parts of absolute alcohol and xylol. Recombine the cover and slide in xylol-balsam directly from this solution.

Item Type: Paper
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > staining techniques
CSHL Authors:
Communities: The Carnegie Institution Department of Genetics
Depositing User: Elizabeth Pessala
Date: April 1929
Date Deposited: 17 Feb 2017 16:10
Last Modified: 17 Feb 2017 16:10
URI: https://repository.cshl.edu/id/eprint/34099

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving