McClintock, Barbara (April 1929) A method for making aceto-carmin smears permanent. Stain Technology, 4 (2). pp. 53-56.
Abstract
Anthers are collected and placed in a solution of 1 part acetic acid to 3 parts of absolute alcohol. The contents of the anther are squeezed out on a slide in a drop of Belling's iron-aceto-carmin solution and a cover glass placed over the drop. Care should be taken to remove all anther walls and flower parts. Heat the slide over an alcohol flame for a second, repeating 4 or 5 times. Place the slide in a petri dish filled with a 10% solution of acetic acid. When the cover glass has risen away from the slide gently remove the cover glass and place in a Coplin jar containing equal parts of alcohol and acetic acid. Likewise, place the slide in this solution. Run both cover and slide thru the following solutions: 1 part acetic acid to 3 parts absolute alcohol, 1 part acetic acid to 9 parts absolute alcohol, absolute alcohol and finally equal parts of absolute alcohol and xylol. Recombine the cover and slide in xylol-balsam directly from this solution.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment Investigative techniques and equipment > staining techniques |
| CSHL Authors: | |
| Communities: | The Carnegie Institution Department of Genetics |
| Depositing User: | Elizabeth Pessala |
| Date: | April 1929 |
| Date Deposited: | 17 Feb 2017 16:10 |
| Last Modified: | 17 Feb 2017 16:10 |
| URI: | https://repository.cshl.edu/id/eprint/34099 |
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