Petes, T. D., Broach, J. R., Wensink, P. C., Hereford, L. M., Fink, G. R., Botstein, D. (September 1978) Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene, 4 (1). pp. 37-49. ISSN 0378-1119 (Print)0378-1119 (Linking)
Abstract
2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA/*genetics DNA, Bacterial/genetics DNA, Recombinant/*isolation & purification Escherichia coli/genetics Nucleic Acid Hybridization Plasmids RNA/genetics RNA, Messenger/genetics RNA, Ribosomal/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/*genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > recombination organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | September 1978 |
| Date Deposited: | 25 Aug 2016 13:50 |
| Last Modified: | 25 Aug 2016 13:50 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33325 |
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