A mutant of Escherichia coli which accumulates large amounts of coproporphyrin

Kazmi, S. A., Bukhari, A. I. (July 1978) A mutant of Escherichia coli which accumulates large amounts of coproporphyrin. Biochim Biophys Acta, 541 (3). pp. 420-4. ISSN 0006-3002 (Print)0006-3002 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/352406http://ww...
DOI: 10.1016/0304-4165(78)90201-5


A mutant of Escherichia coli which accumulates a large amount of coproporphyrin, presumably because of a block in heme biosynthesis, has been isolated after nitrosoguanidine mutagenesis. On rich media, the mutant forms colonies which give bright orange fluorescence when illuminated with ultraviolet light. The mutant appears to be similar to a Salmonella typhimurium mutant, deficient in uroporphyrinogen III cosynthase, described by Sasarman and Desrochers ((1976) J. Bacteriol. 128, 717--721). A striking property of the mutant is that coproporphyrin is retained within the cells in rich media but is almost totally excreted out of cells in minimal glucose medium.

Item Type: Paper
Uncontrolled Keywords: Coproporphyrins/*metabolism Culture Media Escherichia coli/*genetics/growth & development/metabolism Glucose/metabolism Methylnitronitrosoguanidine Mutation Porphyrins/*metabolism
Subjects: organism description > bacteria > escherichia coli
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 3 July 1978
Date Deposited: 14 Oct 2016 19:50
Last Modified: 14 Oct 2016 19:50
Related URLs:
URI: https://repository.cshl.edu/id/eprint/33316

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