Zain, B. S., Roberts, R. J. (March 1978) Characterization and sequence analysis of a recombination site in the hybrid virus Ad2+ND. J Mol Biol, 120 (1). pp. 13-31. ISSN 0022-2836 (Print)0022-2836 (Linking)
Abstract
Restriction endonuclease mapping of an adenovirus-simian virus 40 hybrid virus and adenovirus-2 DNA allowed the characterization of fragments of Ad2+ND1† which contain the two junctions between simian virus (SV40) sequences and adenovirus-2 sequences. The corresponding fragments of Ad2++ DNA were also characterized. One fragment of Ad2+ND1 containing a recombination site, and the corresponding fragment of Ad2++ were analyzed by direct DNA sequence analysis. Comparison of nucleotide sequences in Ad2++, Ad2+ ND1, and SV40 DNAs precisely localized those sequences involved in the final recombination event which produced the stable hybrid virus Ad2+ND1. No sequence homology was detected between the two parent DNAs.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenoviridae Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Viral/*analysis/genetics Electrophoresis Nucleic Acid Hybridization *Recombination, Genetic Simian virus 40 |
| Subjects: | organism description > virus > adenovirus |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Matt Covey |
| Date: | 25 March 1978 |
| Date Deposited: | 13 Dec 2016 15:13 |
| Last Modified: | 13 Dec 2016 15:13 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33299 |
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