The binding site on SV40 DNA for a T antigen-related protein

Tjian, R. (January 1978) The binding site on SV40 DNA for a T antigen-related protein. Cell, 13 (1). pp. 165-79. ISSN 0092-8674 (Print)0092-8674 (Linking)

DOI: 10.1016/0092-8674(78)90147-2


A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.

Item Type: Paper
Uncontrolled Keywords: Adenoviridae *Antigens, Neoplasm *Antigens, Viral Base Sequence Binding Sites DNA Replication DNA, Viral/*metabolism Defective Viruses Deoxyribonucleases/metabolism *Simian virus 40/immunology Viral Proteins/isolation & purification/*metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: January 1978
Date Deposited: 15 Dec 2016 15:20
Last Modified: 15 Dec 2016 15:20
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