Tjian, R. (January 1978) The binding site on SV40 DNA for a T antigen-related protein. Cell, 13 (1). pp. 165-79. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenoviridae *Antigens, Neoplasm *Antigens, Viral Base Sequence Binding Sites DNA Replication DNA, Viral/*metabolism Defective Viruses Deoxyribonucleases/metabolism *Simian virus 40/immunology Viral Proteins/isolation & purification/*metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > virus > SV40 |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | January 1978 |
| Date Deposited: | 15 Dec 2016 15:20 |
| Last Modified: | 15 Dec 2016 15:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33294 |
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