Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R. J. (November 1978) Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae. Nucleic Acids Res, 5 (11). pp. 4055-64. ISSN 0305-1048 (Print)0305-1048 (Linking)
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Abstract
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Avian Myeloblastosis Virus/enzymology Base Sequence *Dna *DNA Restriction Enzymes Deoxyribonucleases Deoxyribonucleotides/analysis Klebsiella pneumoniae/*enzymology Phosphoric Diester Hydrolases RNA-Directed DNA Polymerase |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Matt Covey |
| Date: | November 1978 |
| Date Deposited: | 15 Dec 2016 17:43 |
| Last Modified: | 08 Nov 2017 17:13 |
| PMCID: | PMC342733 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33285 |
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