Graessmann, A., Graessmann, M., Topp, W. C., Botchan, M. (1979) Retransformation of a simian virus 40 revertant cell line, which is resistant to viral and DNA infections, by microinjection of viral DNA. Journal of Virology, 32 (3). pp. 989-994. ISSN 0022-538X
Abstract
We have isolated morphological transformants of cultured cells as dense foci on a monolayer of normal cells appproximately 4 weeks after microinjection of purified simian virus 40 DNA (200 to 400 molecules per cell) directly into the nucleus. Both Rat 1 (an established contact-inhibited rat embryo fibroblast line) and F1' 1--4 (a 5-fluorodeoxyuridine-selected flat revertant from the simian virus 40-transformed 14B cell line) were transformed with an efficiency of 5 to 10% of the cells injected. F1' 1--4 is not susceptible to retransformation by either viral or DNA infection (by calcium phosphate-facilitated cellular uptake), and as a result it had previously been thought to possess a host mutation preventing expression of the simian virus 40 genome.
| Item Type: | Paper |
|---|---|
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell transformation organism description > virus > SV40 |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 1979 |
| Date Deposited: | 24 May 2016 15:29 |
| Last Modified: | 24 May 2016 15:29 |
| PMCID: | PMC525948 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32689 |
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