Garrels, J. I., Schubert, D. (August 1979) Modulation of protein synthesis by nerve growth factor. J Biol Chem, 254 (16). pp. 7978-85. ISSN 0021-9258 (Print)0021-9258 (Linking)
Abstract
The effects of nerve growth factor (NGF) and two analogs of cAMP have been studied in the PC12 line of rat pheochromocytoma cells. We have used two-dimensional gel electrophoresis to detect more than 800 proteins from control and NGF-treated cells. Of these proteins, none were qualitatively repressed in response to NGF, and no new proteins appeared after NGF treatment. Visual inspection of the gels showed that approximately 5% of the proteins were detectably increased or decreased in rate of synthesis by NGF, and each of these changes was mimicked by both cAMP analogs. The two-dimensional gel data were further analyzed by a computerized scanning system. This analysis has revealed many significant changes that are smaller than those detected by eye. Approximately 25 to 30% of the proteins analyzed were found to be altered in rate of synthesis by 30% or more. Statistical analysis has shown that the response to NGF and the response to dibutyryl cAMP are highly correlated, even down to changes as small as 30%. No proteins were found to be significantly altered by both dibutyryl cAMP and 8-bromo cAMP, but not by NGF. These results show that NGF causes only quantitative modulations of protein synthesis in PC12 cells, and these data strongly suggest that the response of PC12 cells to NGF is mediated by cAMP.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Bucladesine/pharmacology Cell Line Cyclic AMP/analogs & derivatives/pharmacology Neoplasm Proteins/*biosynthesis Nerve Growth Factors/*pharmacology Pheochromocytoma Protein Biosynthesis/drug effects Rats |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > Cyclic AMP bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > nerve growth factor |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 25 August 1979 |
| Date Deposited: | 23 May 2016 19:05 |
| Last Modified: | 23 May 2016 19:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32685 |
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