Crawford, L. V., Lane, D. P., Denhardt, D. T., Harlow, E. E., Nicklin, P. M., Osborn, K., Pim, D. C. (1980) Characterization of the complex between SV40 large T antigen and the 53K host protein in transformed mouse cells. Cold Spring Harbor Symposia on Quantitative Biology, 44. pp. 179-187. ISSN 0091-7451
Abstract
Cells transformed by SV40 express the same functions that appear early after lytic infection. The first polypeptide characterized as both a product of the early region of the viral DNA and characteristic of virus-transformed cells was large T antigen. This protein is the product of the A cistron and has an apparent molecular weight of about 94,000 (Rundell et al. 1977). The second polypeptide, small T antigen, was initially detected by in vitro protein synthesis (Greenblatt et al. 1976; Prives et al. 1977). It has a molecular weight of about 20,000 after alkylation (Crawford and O'Farrell 1979), consistent with the estimate of 20,503 for its molecular weight based on the DNA sequence (Fiers et al. 1978; Reddy et al. 1978). Large and small T antigens have common N-terminal sequences (Paucha et al. 1978) and share antigenic determinants (Lane and Robbins 1978).
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > virus > SV40 |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 1980 |
| Date Deposited: | 02 Jun 2016 16:20 |
| Last Modified: | 02 Jun 2016 16:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32674 |
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