Burridge, K., Jordan, L. (November 1979) The glycoproteins of Dictyostelium discoideum. Changes during development. Exp Cell Res, 124 (1). pp. 31-8. ISSN 0014-4827 (Print)0014-4827 (Linking)
Abstract
The glycoproteins of D. discoideum were analyzed by direct binding of radio-iodinated lectins to sodium dodecyl sulfate gels of the successive developmental stages. Compared with the total pattern of proteins, many changes are found in the glycoproteins during development. WGA [wheat germ agglutinin] reacts with few gel bands from the vegetative cells and most of these, including a very intense band at the top of the gel, are lost during the first few hours of development. Approximately halfway through the developmental cycle at least 14 new glycoproteins reacting with WGA begin to appear and progressively accumulate. In contrast, concanavalin A labels many glycoproteins over the complete MW range and most are unaffected during development. Lectins which bind fucose label a single component at the top of the gel of vegetative cells and this decreases rapidly as development begins. No other reactive gel bands are revealed by fucose-binding lectins until the final stages of spore and stalk formation, when 4 high MW glycoproteins are detected. Lectins specific for terminal galactose residues and for N-acetyl-galactosamine, including the intrinsic lectins produced by D. discoideum during its development, failed to reveal any reactive glycoproteins.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cell Differentiation Dictyostelium/cytology/growth & development/*metabolism Glycoproteins/*metabolism Lectins Mannosides/metabolism Molecular Weight Receptors, Concanavalin A/metabolism |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell differentiation |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | November 1979 |
| Date Deposited: | 25 May 2016 20:27 |
| Last Modified: | 25 May 2016 20:27 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32668 |
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