Sadowski, C. L., Henry, R. W., Lobo, S. M., Hernandez, N. (August 1993) Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE. Genes Dev, 7 (8). pp. 1535-48. ISSN 0890-9369 (Print)
Preview |
PDF (Paper)
Hernandez Genes Dev 1993.pdf - Published Version Download (4MB) | Preview |
Abstract
In the human small nuclear RNA (snRNA) promoters, the presence of a TATA box recognized by the TATA box-binding protein (TBP) determines the selection of RNA polymerase III over RNA polymerase II. The RNA polymerase II snRNA promoters are, therefore, good candidates for TBP-independent promoters. We show here, however, that TBP activates transcription from RNA polymerase II snRNA promoters through a non-TATA box element, the snRNA proximal sequence element (PSE), as part of a new snRNA-activating protein complex (SNAPc). In contrast to the previously identified TBP-containing complexes SL1, TFIID, and TFIIIB, which appear dedicated to transcription by a single RNA polymerase, SNAPc is also essential for RNA polymerase III transcription from the U6 snRNA promoter. The U6 initiation complex appears to contain two forms of TBP, one bound to the TATA box and one bound to the PSE as a part of SNAPc, suggesting that multiple TBP molecules can have different functions within a single promoter.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Cross-Linking Reagents DNA-Binding Proteins/ metabolism DNA-Directed RNA Polymerases/ biosynthesis Humans Mice Molecular Sequence Data Promoter Regions (Genetics) Proteins RNA Polymerase II/biosynthesis RNA Polymerase III/biosynthesis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Ribonucleoprotein, U4-U6 Small Nuclear/ genetics Structure-Activity Relationship TATA Box TATA-Box Binding Protein Trans-Activation (Genetics) Trans-Activators/metabolism Transcription Factors/ metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab |
| Depositing User: | Matt Covey |
| Date: | August 1993 |
| Date Deposited: | 19 Apr 2016 15:29 |
| Last Modified: | 03 Nov 2017 20:36 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32516 |
Actions (login required)
 |
Administrator's edit/view item |