Assamunt, N., Mortishiresmith, R. J., Aurora, R., Herr, W., Wright, P. E. (April 1993) The Solution Structure of the Oct-1 Pou-Specific Domain Reveals a Striking Similarity to the Bacteriophage-Lambda Repressor DNA-Binding Domain. Cell, 73 (1). pp. 193-205. ISSN 0092-8674
Abstract
The POU-specific (POU(s)) domain, in association with a POU-type homeodomain, forms the bipartite DNA-binding POU domain. The solution structure of the Oct-1 POU(s) domain has been determined by multidimensional nuclear magnetic resonance spectroscopy and consists of four alpha helices surrounding a conserved hydrophobic core. The POU(s) domain is structurally similar to the DNA-binding domains of the bacteriophage lambda and 434 repressors and 434 Cro. These domains exhibit superimposable helix-turn-helix (HTH) motifs, except that in the POU(s) domain, the first helix and the linker to the second helix of the motif are extended. The conserved structural features have been used to propose a plausible model for DNA binding by the POU(s) domain. A human dwarfism mutation that affects positive control in the related POU domain protein Pit-1 maps to the same region of the HTH motif as do positive control mutations in lambda repressor.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE HEAVY-CHAIN PROMOTER TRANSCRIPTION FACTOR ANTENNAPEDIA HOMEODOMAIN CAENORHABDITIS-ELEGANS PROTEIN CONFORMATIONS IMMUNOGLOBULIN GENES REGULATORY PROTEINS CRYSTAL-STRUCTURE NMR-SPECTROSCOPY |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Matt Covey |
| Date: | April 1993 |
| Date Deposited: | 22 Apr 2016 14:52 |
| Last Modified: | 22 Apr 2016 14:52 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32489 |
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