Isolation and properties of YCK2, a Saccharomyces cerevisiae homolog of casein kinase-1

Vancura, A., O'Connor, A., Patterson, S. D., Mirza, U., Chait, B. T., Kuret, J. (August 1993) Isolation and properties of YCK2, a Saccharomyces cerevisiae homolog of casein kinase-1. Arch Biochem Biophys, 305 (1). pp. 47-53. ISSN 0003-9861 (Print)

DOI: 10.1006/abbi.1993.1391


A soluble fragment of YCK2, a casein kinase-1 isoform from Saccharomyces cerevisiae, has been purified and characterized in vitro. The procedure enriches enzyme activity to a final specific activity of 4.7 mumol min-1 mg-1 (when assayed with casein as substrate). Structural analysis reveals that the preparation arises from N-terminal modification and C-terminal proteolysis of the initially synthesized 546-residue protein, consisting of residues 2-495 +/- 1. Kinetic analysis demonstrates that YCK2 is similar to casein kinase-1 isolated from other organisms in its inability to use GTP as nucleotide substrate, in its sensitivity to heparin and ribofuranosyl-benzimidazole inhibitors, and in its peptide substrate selectivity. The enzyme is unusual, however, in that it is insensitive to the potent mammalian casein kinase-1 inhibitor N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Benzimidazoles/pharmacology Casein Kinase I Casein Kinases Chemistry, Physical Comparative Study Enzyme Stability Guanosine Triphosphate/metabolism Heparin/pharmacology Isoquinolines/pharmacology Kinetics Molecular Sequence Data Protein Kinases/chemistry/ isolation & purification/metabolism Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ enzymology Saccharomyces cerevisiae Proteins Substrate Specificity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
biotechnology > chromatography > protein purification
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Stillman lab
Depositing User: Matt Covey
Date: 15 August 1993
Date Deposited: 21 Apr 2016 18:25
Last Modified: 21 Apr 2016 18:25
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