Berkowitz, L. A., Gilman, M. Z. (July 1990) Two distinct forms of active transcription factor CREB (cAMP response element binding protein). Proc Natl Acad Sci U S A, 87 (14). pp. 5258-62. ISSN 0027-8424 (Print)0027-8424 (Linking)
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Abstract
Mammalian cells express two distinct forms of transcription factor CREB (cAMP response element binding protein) that are apparently the products of alternative splicing of the CREB gene transcript. The two proteins differ by a 14-amino acid serine-rich insertion present in one of the CREB isoforms. We show that both CREB isoforms are expressed in many cell types and mammalian species. Both encode proteins that bind specifically to a cAMP response element in vitro. As expected for proteins of this class, the CREB proteins bind DNA as dimers. Both proteins impart cAMP-regulated transcriptional activity to a heterologous DNA-binding domain, showing that cAMP directly modulates the transcriptional stimulatory activity of CREB. The presence of multiple CREB isoforms with identical DNA-binding specificities but differences in the presumed regulatory domain raises the possibility that CREB proteins may be able to integrate distinct regulatory signals at the level of gene transcription.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cyclic AMP Response Element-Binding Protein DNA/blood/genetics/isolation & purification DNA-Binding Proteins/*genetics/isolation & purification Gene Expression Humans Molecular Sequence Data Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger/genetics T-Lymphocytes/metabolism Transcription Factors/*genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > CREB bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | July 1990 |
| Date Deposited: | 08 Feb 2016 15:58 |
| Last Modified: | 09 Nov 2017 20:40 |
| PMCID: | PMC54302 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32351 |
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