Rice, A. P., Carlotti, F. (April 1990) Mutational analysis of the conserved cysteine-rich region of the human immunodeficiency virus type 1 Tat protein. J Virol, 64 (4). pp. 1864-8. ISSN 0022-538X (Print)0022-538X (Linking)
Abstract
The Tat transactivator protein of human immunodeficiency virus type 1 contains a highly conserved cysteine-rich region, containing seven cysteines from residues 22 through 37. To investigate the importance of noncysteine residues in this region of the Tat protein, we have carried out a mutational analysis, in most cases substituting a single alanine for the wild-type noncysteine residue. Alanine substitution of residue 23, 24, 46, or 47 had no effect on Tat activity in plasmid transfection assays. In contrast, alanine substitutions of all eight noncysteines analyzed, from residues 26 through 41, significantly reduced the activity of the Tat protein, in some cases as drastically as mutations in cysteine residues. The results demonstrate that the precise sequence of the cysteine-rich region is crucial for a fully functional Tat protein.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Cell Line Cysteine Gene Products, tat/*genetics HIV-1/*genetics Humans Molecular Sequence Data Mutation Trans-Activators/*genetics Transfection tat Gene Products, Human Immunodeficiency Virus |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | April 1990 |
| Date Deposited: | 23 Mar 2016 15:58 |
| Last Modified: | 23 Mar 2016 15:58 |
| PMCID: | PMC249332 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32308 |
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