Potashkin, J., Frendewey, D. (February 1990) A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing. EMBO J, 9 (2). pp. 525-34. ISSN 0261-4189 (Print)0261-4189 (Linking)
Abstract
A bank of temperature sensitive (ts-) mutants of Schizosaccharomyces pombe was screened for snRNA expression mutants using an oligodeoxynucleotide that recognizes U2 RNA. One mutant with a novel phenotype was identified that has reduced steady-state levels of the spliceosomal snRNAs U1, U2, U4, U5 and U6. In addition, the mutant exhibits a temperature-dependent accumulation of aberrant U2 and U4 transcripts elongated at their 3' end. The steady-state concentration of the RNA component of RNase P is also reduced in the mutant, whereas the amount of U3 RNA, 7SL RNA, tRNA, rRNA and mRNA are the same as wild-type. Pre-mRNA, pre-tRNA and U6 RNA precursor processing are impaired in the mutant. Genetic analysis demonstrates that the snRNA defects are tightly linked to the ts- growth defect and are recessive. We have named this mutant snm1 to indicate a defect in snRNA maintenance. The data on snm1 suggest that a single trans-acting factor is essential for the maintenance of steady-state levels of several snRNAs and for proper 3' end formation of U2 and U4 RNAs.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Blotting, Northern Gene Expression *Genes, Fungal Molecular Sequence Data *Mutation Oligonucleotide Probes RNA Precursors/genetics RNA Splicing RNA, Catalytic RNA, Fungal/genetics RNA, Ribosomal/genetics RNA, Small Nuclear/*genetics Restriction Mapping Ribonucleases Saccharomycetales/*genetics Schizosaccharomyces/*genetics/growth & development Temperature *Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > analysis and processing > DNA RNA Processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | February 1990 |
| Date Deposited: | 21 Mar 2016 20:21 |
| Last Modified: | 21 Mar 2016 20:21 |
| PMCID: | PMC551696 |
| URI: | https://repository.cshl.edu/id/eprint/32307 |
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