Ratnasabapathy, R., Sheldon, M., Johal, L., Hernandez, N. (December 1990) The HIV-1 long terminal repeat contains an unusual element that induces the synthesis of short RNAs from various mRNA and snRNA promoters. Genes Dev, 4 (12A). pp. 2061-74. ISSN 0890-9369 (Print)0890-9369 (Linking)
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Abstract
We describe an unusual element that activates the synthesis of short transcripts from a wide variety of mRNA and small nuclear RNA (snRNA) promoters, including the U6 RNA polymerase III promoter. This inducer of short transcripts (IST) is located between positions -5 and +82 relative to the cap site in the HIV-1 LTR. In the presence of IST, the total transcriptional activity of the different promoters is greatly increased, but the resulting additional RNA molecules are short, ending around position +60. IST is not the RNA target (TAR) for Tat trans-activation; however, because it relies entirely on cellular factors for activity, IST may serve to provide abundant RNA targets for Tat trans-activation without a requirement for full-length viral mRNA expression.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Genes, tat *HIV Long Terminal Repeat HIV-1/*genetics Molecular Sequence Data Nucleic Acid Conformation *Promoter Regions, Genetic RNA Polymerase II/genetics/metabolism RNA Polymerase III/genetics/metabolism RNA, Messenger/metabolism RNA, Small Nuclear/*genetics RNA, Viral/biosynthesis Restriction Mapping *Transcription, Genetic Transcriptional Activation |
| Subjects: | diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab |
| Depositing User: | Matt Covey |
| Date: | December 1990 |
| Date Deposited: | 28 Mar 2016 19:33 |
| Last Modified: | 03 Nov 2017 20:55 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32293 |
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