Nelson, J. M., Miceli, S. M., Lechevalier, M. P., Roberts, R. J. (April 1990) FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res, 18 (8). pp. 2061-4. ISSN 0305-1048 (Print)0305-1048 (Linking)
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Abstract
A Type II restriction endonuclease, designated FseI, has been partially purified from a Frankia species (NRRL 18528). This enzyme cleaves Adenovirus 2 DNA at three sites, but does not cleave the DNAs from bacteriophages lambda, T7, and phi X174, the animal virus SV40, pUC18 and pBR322. FseI recognizes the octanucleotide sequence 5' GGCCGG decreases CC 3' and cleaves as indicated by the arrow. The frequency of occurrence of FseI sites within sequenced regions of the human genome is similar to that for NotI sites.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes/isolation & purification/*metabolism DNA, Viral/genetics/*metabolism Deoxyribonucleases, Type II Site-Specific/isolation & purification/*metabolism Molecular Sequence Data Oligodeoxyribonucleotides/*metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Matt Covey |
| Date: | 25 April 1990 |
| Date Deposited: | 04 Apr 2016 14:57 |
| Last Modified: | 08 Nov 2017 16:53 |
| PMCID: | PMC330683 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32287 |
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