Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum

Ducommun, B., Tollon, Y., Gares, M., Beach, D., Wright, M. (August 1990) Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum. J Cell Sci, 96 ((Pt 4)). pp. 683-9. ISSN 0021-9533 (Print)0021-9533 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2126544


The regulation of the mitotic histone H1 kinase activity has been analyzed during the naturally synchronous cell cycle of Physarum polycephalum plasmodia. The universal binding property of the p13suc1 Schizosaccharomyces pombe gene product was used to precipitate and assay the cdc2 histone H1 kinase activity. The kinase activity peaks at the beginning of metaphase and its decline, which requires protein synthesis, appears to be an early event during the metaphase process. Microtubular poisons, temperature shifts and DNA synthesis inhibitors were used to perturb cell cycle regulatory pathways and characterize their effects on cdc2 kinase activation. Our results suggest that the full activation of the mitotic kinase requires at least two successive triggering signals involving microtubular components and DNA synthesis.

Item Type: Paper
Uncontrolled Keywords: Aphidicolin Benzimidazoles/pharmacology CDC2 Protein Kinase/isolation & purification/*metabolism *Carbamates *Cell Cycle/drug effects *Cell Cycle Proteins Chromatography, Affinity Cycloheximide/pharmacology DNA, Fungal/biosynthesis DNA-Directed DNA Polymerase/drug effects Diterpenes/pharmacology Fungal Proteins Griseofulvin/pharmacology In Vitro Techniques Microtubules/drug effects/physiology Phosphorylation Physarum *Schizosaccharomyces pombe Proteins Signal Transduction
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: August 1990
Date Deposited: 05 Apr 2016 20:03
Last Modified: 06 Nov 2017 20:12
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32259

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