Morris, G. F., Mathews, M. B. (September 1990) Analysis of the proliferating cell nuclear antigen promoter and its response to adenovirus early region 1. J Biol Chem, 265 (27). pp. 16116-25. ISSN 0021-9258 (Print)0021-9258 (Linking)
Abstract
The levels of the mRNA for the proliferating cell nuclear antigen (PCNA), a DNA replication factor, increase upon growth stimulation of quiescent cells. To study the transcriptional aspect of this response, we have cloned a PCNA gene fragment from size-fractionated human placental DNA. This fragment contains 1269 nucleotides upstream from the PCNA transcriptional start site and includes an Alu sequence that is transcribed in vitro. The PCNA genomic DNA promotes transcription of a linked heterologous reporter gene in HeLa and 293 cells. Transient expression assays and in vitro transcription analyses showed that 249 nucleotides of upstream sequence are sufficient for full promoter activity in HeLa cells, whereas only 172 nucleotides are needed in 293 cells. Co-transfection with a plasmid expressing the adenovirus E1 gene transactivates the PCNA promoter in HeLa cells. An E1-responsive element maps in the 85-nucleotide region immediately upstream of the site of transcription initiation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Adenoviruses, Human/genetics Antigens, Neoplasm/genetics Base Sequence Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular DNA Replication HeLa Cells/metabolism Humans Molecular Sequence Data Nuclear Proteins/*genetics Oligonucleotide Probes Oncogene Proteins, Viral/*metabolism Proliferating Cell Nuclear Antigen *Promoter Regions, Genetic Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic |
| Subjects: | organism description > virus > adenovirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Proliferating Cell Nuclear Antigen bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 25 September 1990 |
| Date Deposited: | 06 Apr 2016 16:07 |
| Last Modified: | 06 Apr 2016 16:07 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32251 |
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