stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe

Hudson, J. D., Feilotter, H., Lingner, C., Rowley, R., Young, P. G. (1991) stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe. Cold Spring Harb Symp Quant Biol, 56. pp. 599-604. ISSN 0091-7451 (Print)0091-7451 (Linking)

DOI: 10.1101/SQB.1991.056.01.068


A novel element in the mitotic control, stf1, has been identified genetically by its ability to rescue cdc25-22 as well as a gene disruption of cdc25. This is the first phenotypically non-wee mutation shown to do so. stf1-1 functions additively with cdc2-1w, cdc2-3w, or wee1-6 to rescue cdc25. The available data are consistent with the wild-type gene product operating either on the same pathway as cdc25 or to stimulate cdc2 by a pathway independent of cdc25 or wee1. The stf1 gene has been cloned and sequenced and encodes a putative protein of 50-65 kD, depending on whether a potential intron is present. It is a novel protein with no homology detected in the current data bases. When challenged with hydroxyurea, stf1-1 acts additively with cdc2-3w in rescuing cdc25 mutants and in allowing mitosis to occur without DNA synthesis. It does not appear to play a role in the nutritional sensing pathway nor in the pathway mediating radiation-induced G2 delay.

Item Type: Paper
Additional Information: Hudson, J D Feilotter, H Lingner, C Rowley, R Young, P G eng CA-40245/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1991/01/01 Cold Spring Harb Symp Quant Biol. 1991;56:599-604.
Uncontrolled Keywords: Alleles *Cell Cycle Proteins DNA, Fungal/biosynthesis/drug effects Fungal Proteins/*genetics Gene Expression Regulation, Fungal *Genes, Fungal Hydroxyurea/pharmacology Mitosis/*genetics/radiation effects Mutation *Nuclear Proteins Protein Kinases/genetics *Protein-Tyrosine Kinases Schizosaccharomyces/cytology/*genetics Schizosaccharomyces pombe Proteins Suppression, Genetic *ras-GRF1
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1991
Date Deposited: 09 Dec 2015 21:06
Last Modified: 09 Dec 2015 21:06
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