Stable growth of simian virus 40 recombinants containing multimerized enhancers

Ondek, B., Herr, W. (March 1991) Stable growth of simian virus 40 recombinants containing multimerized enhancers. J Virol, 65 (3). pp. 1596-9. ISSN 0022-538X (Print)0022-538X (Linking)



Multiple copies of each of three genetically defined simian virus 40 protoenhancers, A, B, and C, were able to substitute for the wild-type simian virus 40 enhancer. Although the recombinant viruses grew poorly, they could be propagated without the accumulation of enhancer rearrangements that might improve viability. Mutations that inactivate the multimerized B and C protoenhancers abolished virus growth, but, unexpectedly, a mutation that inactivates the octamer-enhanson within the B protoenhancer increased virus viability. This positive effect may reflect loss of repression of the B protoenhancer by the ubiquitous octamer-motif-binding protein Oct-1.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Cell Line *Enhancer Elements, Genetic Molecular Sequence Data Recombination, Genetic Restriction Mapping Simian virus 40/*genetics/growth & development
Subjects: organism description > virus
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: March 1991
Date Deposited: 10 Dec 2015 21:51
Last Modified: 10 Dec 2015 21:51
PMCID: PMC239944
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