Ondek, B., Herr, W. (March 1991) Stable growth of simian virus 40 recombinants containing multimerized enhancers. J Virol, 65 (3). pp. 1596-9. ISSN 0022-538X (Print)0022-538X (Linking)
Abstract
Multiple copies of each of three genetically defined simian virus 40 protoenhancers, A, B, and C, were able to substitute for the wild-type simian virus 40 enhancer. Although the recombinant viruses grew poorly, they could be propagated without the accumulation of enhancer rearrangements that might improve viability. Mutations that inactivate the multimerized B and C protoenhancers abolished virus growth, but, unexpectedly, a mutation that inactivates the octamer-enhanson within the B protoenhancer increased virus viability. This positive effect may reflect loss of repression of the B protoenhancer by the ubiquitous octamer-motif-binding protein Oct-1.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Cell Line *Enhancer Elements, Genetic Molecular Sequence Data Recombination, Genetic Restriction Mapping Simian virus 40/*genetics/growth & development |
| Subjects: | organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Matt Covey |
| Date: | March 1991 |
| Date Deposited: | 10 Dec 2015 21:51 |
| Last Modified: | 10 Dec 2015 21:51 |
| PMCID: | PMC239944 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32152 |
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