Human D-type cyclin

Xiong, Y., Connolly, T., Futcher, B., Beach, D. (May 1991) Human D-type cyclin. Cell, 65 (4). pp. 691-9. ISSN 0092-8674 (Print)0092-8674 (Linking)

DOI: 10.1016/0092-8674(91)90100-D


A cDNA library prepared from a human glioblastoma cell line has been introduced into a budding yeast strain that lacks CLN1 and CLN2 and is conditionally deficient for CLN3 function. We rescued a gene that we call cyclin D1. It is related to A-, B-, and CLN-type cyclins, but appears to define a new subclass within the cyclin gene family. Transcription of the cyclin D1 gene gives rise to two major transcripts through alternative polyadenylation. The cyclin D1 gene transcript and its 34 kd product are both abundant in the glioblastoma cell line of origin.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Biological Evolution Blotting, Western Cell Line Cloning, Molecular/methods Cyclins/*genetics DNA, Neoplasm/genetics/isolation & purification Gene Library Genes Glioma HeLa Cells/physiology Humans Molecular Sequence Data RNA, Neoplasm/genetics/isolation & purification Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
CSHL Authors:
Communities: CSHL labs > Beach lab
CSHL labs > Futcher lab
Depositing User: Matt Covey
Date: 17 May 1991
Date Deposited: 06 Jan 2016 18:06
Last Modified: 06 Jan 2016 18:06
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