Ducommun, B., Brambilla, P., Felix, M. A., Franza, B. R., Karsenti, E., Draetta, G. (November 1991) cdc2 phosphorylation is required for its interaction with cyclin. Embo Journal, 10 (11). pp. 3311-3319. ISSN 0261-4189
Abstract
Activation of the cdc2 protein kinase at different stages of the cell cycle is regulated by post-translational modifications and interactions with cyclins. We show that in vitro translated human cdc2 binds very poorly to A and B cyclins, unless it has been preincubated with a Xenopus egg extract. This results in the phosphorylation of cdc2 which allows binding to cyclins. The replacement of Thr161, a residue conserved and phosphorylated in other protein kinases, with valine inhibits cdc2 association with A and B cyclins. In addition, mutations in the amino-terminus of cdc2 and within the conserved 'PSTAIR'region strongly inhibit binding. The Thr161Val mutation causes a lethal phenotype in the fission yeast Schizosacharomyces pombe, while replacement of Thr161 with glutamic acid, potentially mimicking phosphorylation, causes uncoordination of mitosis and multiple cytokinesis. These results suggest that a threonine phosphorylation/dephosphorylation cycle is involved in regulating cdc2 function.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | cdc2 protein kinase cyclin phosphorylation dependent protein-kinase alanine-scanning mutagenesis cell-cycle fission yeast tyrosine phosphorylation h1 kinase activation xenopus gene dephosphorylation |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | November 1991 |
| Date Deposited: | 14 Jan 2016 17:52 |
| Last Modified: | 14 Jan 2016 17:52 |
| PMCID: | PMC453057 |
| URI: | https://repository.cshl.edu/id/eprint/32046 |
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