Hydroxyproline O-arabinosyltransferase mutants oppositely alter tip growth in Arabidopsis thaliana and Physcomitrella patens

MacAlister, C. A., Ortiz-Ramirez, C., Becker, J. D., Feijo, J. A., Lippman, Z. B. (January 2016) Hydroxyproline O-arabinosyltransferase mutants oppositely alter tip growth in Arabidopsis thaliana and Physcomitrella patens. Plant J, 85 (2). pp. 193-208. ISSN 1365-313X (Electronic)0960-7412 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/26577059
DOI: 10.1111/tpj.13079


Hydroxyproline O-arabinosylatransferases (HPATs) are members of a small, deeply conserved family of plant-specific glycosyltransferases that add arabinose sugars to diverse proteins including cell wall-associated extensins and small signaling peptides. Recent genetic studies in flowering plants suggest that different HPAT homologs have been co-opted to function in diverse species-specific developmental contexts. However, nothing is known about the roles of HPATs in basal plants. We show that complete loss of HPAT function in Arabidopsis thaliana and the moss Physcomitrella patens results in a shared defect in gametophytic tip cell growth. Arabidopsis hpat1/2/3 triple knockout mutants suffer from a strong male sterility defect as a consequence of pollen tubes that fail to fully elongate following pollination. Knocking out the two HPAT genes of Physcomitrella results in larger multicellular filamentous networks due to increased elongation of protonemal tip cells. Physcomitrella hpat mutants lack cell-wall associated Hyp-arabinosides, can be rescued with exogenous cellulose, and global expression profiling shows that cell wall-associated genes are severely misexpressed, implicating a defect in cell wall formation during tip growth. Our findings point to a major role for HPATs in influencing cell elongation during tip growth in plants. This article is protected by copyright. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis thaliana Physcomitrella patens arabinosylation cell wall development extensins glycosylation pollination protonema tip growth
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics
organism description > plant
CSHL Authors:
Communities: CSHL labs > Lippman lab
Depositing User: Matt Covey
Date: 18 January 2016
Date Deposited: 19 Nov 2015 20:39
Last Modified: 27 Jan 2017 21:27
PMCID: PMC4738400
Related URLs:
URI: https://repository.cshl.edu/id/eprint/31997

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving