Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J. W., Elledge, S., Nishimoto, T., Morgan, D. O., Franza, B. R., Roberts, J. M. (September 1992) Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science, 257 (5077). pp. 1689-94. ISSN 0036-8075 (Print)0036-8075 (Linking)
Abstract
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals B-Lymphocytes/metabolism *CDC2-CDC28 Kinases Cell Line Cyclin-Dependent Kinase 2 *Cyclin-Dependent Kinases Cyclins/*metabolism Flow Cytometry G1 Phase/*physiology Humans Immunoblotting Immunosorbent Techniques Protein Kinases/*metabolism *Protein-Serine-Threonine Kinases Rats |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 18 September 1992 |
| Date Deposited: | 18 Sep 2015 19:01 |
| Last Modified: | 18 Sep 2015 19:01 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31865 |
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