Tanaka, M., Lai, J. S., Herr, W. (February 1992) Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter. Cell, 68 (4). pp. 755-67. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA binding specificity but instead through the use of promoter-selective activation domains. These distinct activation domains lead to stimulation of the U2 small nuclear RNA promoter by Oct-1 and an mRNA promoter by Oct-2. An Oct-2 variant, called Oct-2B, differs from Oct-2 by an Oct-1-related C-terminal extension that results from alternative splicing. This variant gains the ability to activate the U2 small nuclear RNA promoter. Thus, the promoter selectivity of a transcriptional activator can be changed, in this case by alternative splicing, without affecting its DNA binding specificity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA-Binding Proteins/genetics Gene Expression Regulation HeLa Cells Humans Models, Genetic *Promoter Regions, Genetic RNA Splicing RNA, Messenger/*genetics RNA, Small Nuclear/*genetics Trans-Activators/*genetics Transfection |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Matt Covey |
| Date: | 21 February 1992 |
| Date Deposited: | 01 Oct 2015 20:05 |
| Last Modified: | 01 Oct 2015 20:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31781 |
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