Lobo, S. M., Tanaka, M., Sullivan, M. L., Hernandez, N. (December 1992) A Tbp Complex Essential for Transcription from Tata-Less but Not Tata-Containing Rna Polymerase-Iii Promoters Is Part of the Tfiiib Fraction. Cell, 71 (6). pp. 1029-1040. ISSN 0092-8674
Abstract
The TATA box-binding protein TBP directs transcription by all three eukaryotic RNA polymerases. In mammalian cells, TBP is found in at least three different complexes: SL1, D-TFIID, and B-TFIID. While SL1 and D-TFIID are involved in RNA polymerase I and II transcription, respectively, no unique function has been assigned to the B-TFIID complex. Here we show that the TFIIIB fraction required for RNA polymerase III transcription contains two separable components, one of which is a TBP-containing complex that may correspond to B-TFIID. For transcription of TATA-less RNA polymerase III genes such as the VAI, 5S, and 7SL genes, this complex cannot be replaced by either TBP alone or the D-TFIID complex. In contrast, TBP alone is active for basal transcription from the TATA-containing U6 promoter. This indicates different requirements for recruiting TBP to TATA-less and TATA-containing RNA polymerase III promoters.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | HUMAN U6 GENE SACCHAROMYCES-CEREVISIAE ACCURATE TRANSCRIPTION MULTIPLE FACTORS BINDING-PROTEIN SNRNA PROMOTER FACTOR-TAU SCHIZOSACCHAROMYCES-POMBE FUNCTIONAL DOMAINS FACTOR-EPSILON |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab |
| Depositing User: | Matt Covey |
| Date: | December 1992 |
| Date Deposited: | 01 Oct 2015 19:40 |
| Last Modified: | 01 Oct 2015 19:40 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31778 |
Actions (login required)
 |
Administrator's edit/view item |